Molecular Assays
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Gene expression analysis
- Robust validated technology
- Quantigene: Multi-plex and Single-plex gene assays
- Many advantages over the traditional qPCR
- Cost and time effective
Multi-plex Gene assay - Unique Benefits
- Sample types – Whole blood, FFPE, cells, serum, tissue lysates, no RNA purification required
- Specific Probes – Each gene is specifically targeted by 6 different probes designed along the target
- True amplification – The assay is based on branched DNA technology (sequential nucleic acid
- High precision – Coefficient of variation (CV) of less than 20% for entire process (sample isolation to results)
- Multiplexing of targets – Up to 50 targets per well, housekeeping included
- Multiple target species – Large inventory of validated genes for various species
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Single-Plex Gene assay - Unique Benefits
- Sample types – Whole blood, FFPE, cells, serum, tissue lysates, no RNA purification required
- True amplification – The assay is based on branched DNA technology (sequential nucleic acid hybridization)
- Easy workflow – Minimum sample preparation and ELISA like workflow
- High precision – Coefficient of variation (CV) of less than 10% for entire process (sample isolation to results)
Target validation using siRNA knock-down.
HeLa cells were transfected with siNegative Control and siWRN. The WRN gene expression was then assayed 72 hours later to confirm siRNA-mediated knock-down. Positive control: K562 cells; HeLa cells = un-transfected cells
ANOVA one-way confirmed significant difference in WRN expression between siNegative Control and siWRN (*p<0.05); and K562 (****p<0.0001) [n=3 ± SEM].
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